Ligand-responsive RNA mechanical switches.
RNA Biol
; 12(8): 780-6, 2015.
Article
en En
| MEDLINE
| ID: mdl-26158858
ABSTRACT
Ligand-responsive RNA mechanical switches represent a new class of simple switching modules that adopt well-defined ligand-free and bound conformational states, distinguishing them from metabolite-sensing riboswitches. Initially discovered in the internal ribosome entry site (IRES) of hepatitis C virus (HCV), these RNA switch motifs were found in the genome of diverse other viruses. Although large variations are seen in sequence and local secondary structure of the switches, their function in viral translation initiation that requires selective ligand recognition is conserved. We recently determined the crystal structure of an RNA switch from Seneca Valley virus (SVV) which is able to functionally replace the switch of HCV. The switches from both viruses recognize identical cognate ligands despite their sequence dissimilarity. Here, we describe the discovery of 7 new switches in addition to the previously established 5 examples. We highlight structural and functional features unique to this class of ligand-responsive RNA mechanical switches and discuss implications for therapeutic development and the construction of RNA nanostructures.
Palabras clave
AEV, avian encephalomyelitis virus; BDV, border disease virus; BVDV, bovine viral diarrhea virus; CSFV, classical swine fever virus; DHV, Duck hepatitis virus; DPV, duck picornavirus; GBV, GB virus; GPV, giraffe pestivirus; HCV, hepatitis C virus; IRES; IRES, internal ribosome entry site; IVT, in vitro translation; NPHV, non-primate hepacivirus; RNA switch; SPV, simian picornavirus; SVV, Seneca Valley virus; conformational switch; hepatitis C virus; riboswitch
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_diarrhea
Asunto principal:
ARN
/
ARN Viral
/
Riboswitch
/
Conformación de Ácido Nucleico
Idioma:
En
Revista:
RNA Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2015
Tipo del documento:
Article