[Effects of polydatin on learning and memory and Cdk5 kinase activity in the hippocampus of rats with chronic alcoholism].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 31(2): 117-20, 2015 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-26248414
ABSTRACT
OBJECTIVE:
To observe the effects of polydatin on learning and memory and cyclin-dependent kinase 5 (Cdk5) kinase activity in the hippocampus of rats with chronic alcoholism.METHODS:
Forty rats were randomly divided into 4 groups control group, chronic alcoholism group, low and high polydatin group. The rat chronic alcoholism model was established by ethanol 3.0 g/(kg · d) (intragastric administration). The abstinence scoring was used to evaluate the rats withdrawal symptoms; cognitive function was measured by Morris water maze experiment; Cdk5 protein expression in the hippocampus was detected by immunofluorescence; Cdk5 kinase activity in the hippocampus was detected by liquid scintillation counting method.RESULTS:
The abstinence score, escape latency, Cdk5 kinase activity in chronic alcoholism group rats were significantly higher than those of control group (P < 0.05). The abstinence score, escape latency in high polydatin group rats were significantly lower than those of chronic alcoholism group (P < 0.05); Cdk5 kinase activity in high and low polydatin group rats was significantly lower than that of chronic alcoholism group( P < 0.05); immunofluorescence showed that the Cdk5 positive cells of chronic alcoholism group were significantly increased compared with control group (P < 0.05), and the Cdk5 positive cells of polydatin groups were significantly decreased compared with chronic alcoholism group ( P < 0.05).CONCLUSION:
Polydatin-reduced the chronic alcoholism damage may interrelate with regulation of Cdk5 kinase activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estilbenos
/
Alcoholismo
/
Quinasa 5 Dependiente de la Ciclina
/
Glucósidos
/
Hipocampo
/
Aprendizaje
/
Memoria
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Asunto de la revista:
FISIOLOGIA
Año:
2015
Tipo del documento:
Article