Improved quantification of protein in vaccines containing aluminum hydroxide by simple modification of the Lowry method.
Vaccine
; 33(39): 5031-4, 2015 09 22.
Article
en En
| MEDLINE
| ID: mdl-26275477
ABSTRACT
Aluminum (Al) components in vaccines are known to act as adsorbents that interfere with accurate protein quantification by the Lowry method. Therefore, certain modifications based on the characteristics and compositions of the vaccine are required for determination of protein contents. We investigated the effects of an additional centrifugal separation and found that protein contents were overestimated by up to 238% without centrifugation through a collaborative study performed with hepatitis B vaccines containing Al. However, addition of a centrifugation step yielded protein concentrations that were similar to the actual values, with small coefficients of variation (CVs). Proficiency testing performed in 11 laboratories showed that four laboratories did not have satisfactory results for vaccines containing aluminum hydroxide, although all laboratories were proficient in protein analysis when samples did not contain aluminum hydroxide. Incomplete resuspension of aluminum hydroxide solution with alkaline copper solution was the major cause of insufficient proficiency in these laboratories.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Técnicas de Química Analítica
/
Vacunas contra Hepatitis B
/
Hidróxido de Aluminio
Tipo de estudio:
Clinical_trials
Idioma:
En
Revista:
Vaccine
Año:
2015
Tipo del documento:
Article