On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
Dev Cell
; 35(3): 383-94, 2015 Nov 09.
Article
en En
| MEDLINE
| ID: mdl-26555057
ABSTRACT
A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of â¼10,000 proteins and â¼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Xenopus laevis
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ARN Mensajero
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Regulación del Desarrollo de la Expresión Génica
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Proteínas de Xenopus
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Desarrollo Embrionario
Límite:
Animals
Idioma:
En
Revista:
Dev Cell
Asunto de la revista:
EMBRIOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos