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Chromatin Dynamics and the RNA Exosome Function in Concert to Regulate Transcriptional Homeostasis.
Rege, Mayuri; Subramanian, Vidya; Zhu, Chenchen; Hsieh, Tsung-Han S; Weiner, Assaf; Friedman, Nir; Clauder-Münster, Sandra; Steinmetz, Lars M; Rando, Oliver J; Boyer, Laurie A; Peterson, Craig L.
Afiliación
  • Rege M; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
  • Subramanian V; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Zhu C; Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
  • Hsieh TH; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
  • Weiner A; School of Computer Science and Engineering, The Hebrew University, Jerusalem 91904, Israel; Alexander Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
  • Friedman N; School of Computer Science and Engineering, The Hebrew University, Jerusalem 91904, Israel; Alexander Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
  • Clauder-Münster S; Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany.
  • Steinmetz LM; Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Rando OJ; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
  • Boyer LA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Peterson CL; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address: craig.peterson@umassmed.edu.
Cell Rep ; 13(8): 1610-22, 2015 Nov 24.
Article en En | MEDLINE | ID: mdl-26586442
The histone variant H2A.Z is a hallmark of nucleosomes flanking promoters of protein-coding genes and is often found in nucleosomes that carry lysine 56-acetylated histone H3 (H3-K56Ac), a mark that promotes replication-independent nucleosome turnover. Here, we find that H3-K56Ac promotes RNA polymerase II occupancy at many protein-coding and noncoding loci, yet neither H3-K56Ac nor H2A.Z has a significant impact on steady-state mRNA levels in yeast. Instead, broad effects of H3-K56Ac or H2A.Z on RNA levels are revealed only in the absence of the nuclear RNA exosome. H2A.Z is also necessary for the expression of divergent, promoter-proximal noncoding RNAs (ncRNAs) in mouse embryonic stem cells. Finally, we show that H2A.Z functions with H3-K56Ac to facilitate formation of chromosome interaction domains (CIDs). Our study suggests that H2A.Z and H3-K56Ac work in concert with the RNA exosome to control mRNA and ncRNA expression, perhaps in part by regulating higher-order chromatin structures.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Cromatina / Complejo Multienzimático de Ribonucleasas del Exosoma / Homeostasis Límite: Animals Idioma: En Revista: Cell Rep Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Cromatina / Complejo Multienzimático de Ribonucleasas del Exosoma / Homeostasis Límite: Animals Idioma: En Revista: Cell Rep Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
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