Autophagy deficiency in ß cells blunts incretin-induced suppression of glucagon release from α cells.
Islets
; 7(5): e1129096, 2015.
Article
en En
| MEDLINE
| ID: mdl-26744903
ABSTRACT
Incretin-based therapy such as GLP-1 receptor agonists and DPP-4 inhibitors for type 2 diabetes mellitus is characterized by glucose-dependent insulin secretion and glucose-inhibited glucagon secretion. Recently, autophagy deficiency in islet ß cells has been shown to contribute to the pathogenesis of type 2 diabetes mellitus however, with the role of incretin has not been established. To evaluate the role of autophagy in incretin effects, 8-week-old male ß cell-specific Atg7 knockout (Atg7(Δß cell)) mice and wild-type mice were administered vildagliptin for 12 weeks. Vildagliptin treatment improved glucose intolerance and hypoinsulinemia; however, it failed to suppress serum glucagon levels after glucose loading in the Atg7(Δß cell) mice. Ex vivo glucose-induced glucagon suppression was also blunted in the islets from vildagliptin-treated Atg7(Δß cell) mice. The α cell mass was not affected by ß cell autophagy deficiency or vildagliptin. However, glucagon mRNA expression was significantly increased by vildagliptin in the autophagy-deficient islets, and was significantly reduced by vildagliptin in wild-type islets. Pancreatic glucagon contents were not in agreement with the changes in mRNA expression, suggesting a dysregulation in glucagon translation and secretion. In vitro studies revealed that glucose-stimulated cAMP production was impaired in the autophagy-deficient islets exposed to exendin-4. Taken together, the results suggest that the constitutive autophagy in ß cells could regulate incretin-induced glucagon expression and release in α cells, and that cAMP may play a role in this process.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autofagia
/
Glucagón
/
Islotes Pancreáticos
/
Diabetes Mellitus Experimental
/
Células Secretoras de Glucagón
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Células Secretoras de Insulina
/
Incretinas
Límite:
Animals
Idioma:
En
Revista:
Islets
Asunto de la revista:
ENDOCRINOLOGIA
/
GASTROENTEROLOGIA
Año:
2015
Tipo del documento:
Article