Bacterial culture detection and identification in blood agar plates with an optoelectronic nose.
Analyst
; 141(3): 918-25, 2016 Feb 07.
Article
en En
| MEDLINE
| ID: mdl-26753182
ABSTRACT
Clinical microbiology automation is currently limited by the lack of an in-plate culture identification system. Using an inexpensive, printed, disposable colorimetric sensor array (CSA) responsive to the volatiles emitted into plate headspace by microorganisms during growth, we report here that not only the presence but the species of bacteria growing in plate was identified before colonies are visible. In 1894 trials, 15 pathogenic bacterial species cultured on blood agar were identified with 91.0% sensitivity and 99.4% specificity within 3 hours of detection. The results indicate CSAs integrated into Petri dish lids present a novel paradigm to speciate microorganisms, well-suited to integration into automated plate handling systems.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Sonda Molecular
/
Compuestos Orgánicos Volátiles
/
Nariz Electrónica
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Analyst
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos