Extended-spectrum ß-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay.
J Infect Dev Ctries
; 10(1): 43-52, 2016 Jan 31.
Article
en En
| MEDLINE
| ID: mdl-26829536
ABSTRACT
INTRODUCTION:
To characterize extended-spectrum ß-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals.METHODOLOGY:
Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates.RESULTS:
Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6')Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated.CONCLUSIONS:
The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Beta-Lactamasas
/
Quinolonas
/
Farmacorresistencia Bacteriana
/
Factores de Virulencia
/
Escherichia coli
/
Infecciones por Escherichia coli
/
Genotipo
Límite:
Humans
País/Región como asunto:
America do sul
/
Uruguay
Idioma:
En
Revista:
J Infect Dev Ctries
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2016
Tipo del documento:
Article
País de afiliación:
Uruguay