Analytical detection and characterization of biopharmaceutical glycosylation by MS.
Bioanalysis
; 8(7): 711-27, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26964748
ABSTRACT
Glycosylation plays an important role in ensuring the proper structure and function of most biotherapeutic proteins. Even small changes in glycan composition, structure, or location can have a drastic impact on drug safety and efficacy. Recently, glycosylation has become the subject of increased focus as biopharmaceutical companies rush to create not only biosimilars, but also biobetters based on existing biotherapeutic proteins. Against this backdrop of ongoing biopharmaceutical innovation, updated methods for accurate and detailed analysis of protein glycosylation are critical for biopharmaceutical companies and government regulatory agencies alike. This review summarizes current methods of characterizing biopharmaceutical glycosylation, including compositional mass profiling, isomer-specific profiling and structural elucidation by MS and hyphenated techniques.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Glicopéptidos
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Biosimilares Farmacéuticos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2016
Tipo del documento:
Article