[Distribution of Formic Acid after Methanol Intoxication in Rats].
Fa Yi Xue Za Zhi
; 31(6): 450-3, 2015 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-27141803
ABSTRACT
OBJECTIVE:
To investigate concentration and distribution in blood and tissues of formic acid after methanol intoxication in rats.METHODS:
The Sprague-Dawley rats were divided into groups for control group and 3-day and 7-day intoxication treatment groups. The experimental groups were administered methanol by gavage with the initial dose of 8 mL/kg and followed with 4 mL/kg supplemental dose 24 h later. After 3 days and 7 days later, rats were killed by decapitation. Then samples of cardiac blood, liver, kidney, brain, heart and stomach of each group were collected. Formic acid concentrations were detected by high performance liquid chromatography.RESULTS:
Formic acid concentrations in tissues were higher than in blood. Compared with 3-day intoxication group, there was an increase formic acid of concentration in brain and stomach in 7-day intoxication group, while a decrease in liver and kidney (P < 0.05).CONCLUSION:
High performance liquid chromatography could be used to accurately detect formic acid. As the metabolite of methanol, formic acid accumulates in rat blood and tissues after intoxication and the concentrations in organs and tissues are obviously higher than in blood.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Metanol
/
Formiatos
Límite:
Animals
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2015
Tipo del documento:
Article