Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development.
Dev Biol
; 414(2): 133-41, 2016 06 15.
Article
en En
| MEDLINE
| ID: mdl-27158028
ABSTRACT
It is now becoming evident that hydrogen peroxide (H2O2), which is constantly produced by nearly all cells, contributes to bona fide physiological processes. However, little is known regarding the distribution and functions of H2O2 during embryonic development. To address this question, we used a dedicated genetic sensor and revealed a highly dynamic spatio-temporal pattern of H2O2 levels during zebrafish morphogenesis. The highest H2O2 levels are observed during somitogenesis and organogenesis, and these levels gradually decrease in the mature tissues. Biochemical and pharmacological approaches revealed that H2O2 distribution is mainly controlled by its enzymatic degradation. Here we show that H2O2 is enriched in different regions of the developing brain and demonstrate that it participates to axonal guidance. Retinal ganglion cell axonal projections are impaired upon H2O2 depletion and this defect is rescued by H2O2 or ectopic activation of the Hedgehog pathway. We further show that ex vivo, H2O2 directly modifies Hedgehog secretion. We propose that physiological levels of H2O2 regulate RGCs axonal growth through the modulation of Hedgehog pathway.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Ganglionares de la Retina
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Pez Cebra
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Proteínas de Pez Cebra
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Proteínas Hedgehog
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Neurogénesis
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Orientación del Axón
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Peróxido de Hidrógeno
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Dev Biol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Francia