Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata).
J Microencapsul
; 33(4): 344-54, 2016 Jun.
Article
en En
| MEDLINE
| ID: mdl-27292913
Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 µg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1-1), KLGPKGPR (T1-2), and SSPGPPVH (T2-1). By database search, T2-1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 µg/mL. Then, T1 and T2-1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2-1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Tortugas
/
Quitosano
/
Nanocápsulas
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Antineoplásicos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Microencapsul
Asunto de la revista:
FARMACIA
Año:
2016
Tipo del documento:
Article