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Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain.
Lake, Blue B; Ai, Rizi; Kaeser, Gwendolyn E; Salathia, Neeraj S; Yung, Yun C; Liu, Rui; Wildberg, Andre; Gao, Derek; Fung, Ho-Lim; Chen, Song; Vijayaraghavan, Raakhee; Wong, Julian; Chen, Allison; Sheng, Xiaoyan; Kaper, Fiona; Shen, Richard; Ronaghi, Mostafa; Fan, Jian-Bing; Wang, Wei; Chun, Jerold; Zhang, Kun.
Afiliación
  • Lake BB; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
  • Ai R; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
  • Kaeser GE; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA. Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA.
  • Salathia NS; Illumina, San Diego, CA, USA.
  • Yung YC; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA.
  • Liu R; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
  • Wildberg A; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
  • Gao D; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
  • Fung HL; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
  • Chen S; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
  • Vijayaraghavan R; Illumina, San Diego, CA, USA.
  • Wong J; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA.
  • Chen A; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA.
  • Sheng X; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA.
  • Kaper F; Illumina, San Diego, CA, USA.
  • Shen R; Illumina, San Diego, CA, USA.
  • Ronaghi M; Illumina, San Diego, CA, USA.
  • Fan JB; Illumina, San Diego, CA, USA. kzhang@bioeng.ucsd.edu jchun@scripps.edu wei-wang@ucsd.edu jianbing_fan@yahoo.com.
  • Wang W; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA. kzhang@bioeng.ucsd.edu jchun@scripps.edu wei-wang@ucsd.edu jianbing_fan@yahoo.com.
  • Chun J; Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA, USA. kzhang@bioeng.ucsd.edu jchun@scripps.edu wei-wang@ucsd.edu jianbing_fan@yahoo.com.
  • Zhang K; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA. kzhang@bioeng.ucsd.edu jchun@scripps.edu wei-wang@ucsd.edu jianbing_fan@yahoo.com.
Science ; 352(6293): 1586-90, 2016 Jun 24.
Article en En | MEDLINE | ID: mdl-27339989
ABSTRACT
The human brain has enormously complex cellular diversity and connectivities fundamental to our neural functions, yet difficulties in interrogating individual neurons has impeded understanding of the underlying transcriptional landscape. We developed a scalable approach to sequence and quantify RNA molecules in isolated neuronal nuclei from a postmortem brain, generating 3227 sets of single-neuron data from six distinct regions of the cerebral cortex. Using an iterative clustering and classification approach, we identified 16 neuronal subtypes that were further annotated on the basis of known markers and cortical cytoarchitecture. These data demonstrate a robust and scalable method for identifying and categorizing single nuclear transcriptomes, revealing shared genes sufficient to distinguish previously unknown and orthologous neuronal subtypes as well as regional identity and transcriptomic heterogeneity within the human brain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Science Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Science Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos