[Protective Effects of Different Extracts of Imperatae Rhizoma in Rats with Adriamycin Nephrosis and Influence on Expression of TGF-ß1, and NF-κB p65].

OBJECTIVE: To study the protective effect of Imperatae Rhizoma extract on renal tissues in rats with Adriamycin nephrosis, and to explore the possible mechanism. METHODS: The nephrosis model was induced by adriamycin 6.5 mg/kg intravenously in rats. The rats were randomly divided into seven groups, including normal group, model group, predisone group, petroleum ether groups, ethyl acetate group ,alcohol group, and water parts group, and treated for eight weeks. The protein content of 24 hours urine excretion was tested respectively by automatic biochemistry analyzer each week. After eight weeks, BUN, CRE, TCHO, TG, TP and ALB in serum were examined by automatic biochemistry analyzer. The TNF-α in serum was measured by ELISA. The expression of TGF-ß1, and NF-κB p65 in renal tissues were detected by immunohistochemistry respectively. The pathological changes in the renal were observed by light microscope. RESULTS: Compared with the model group ,the proteinuria of the rats in ethyl acetate group obviously reduced at the 6th, 7th, 8th week, the content of TNF-α in serum and the expression of TGF-ß1, and NF-κB p65 in renal tissues significantly reduced, but the content of TP and ALB in serum were increased obviously. In the alcohol and water parts groups ,the level of TG in serum and the protein content of 24 hours urine excretion of the 6th and 7th week were significantly decreased. The ethyl acetate, alcohol and water parts groups ameliorated the changes of pathology in renal. CONCLUSION: The different extracts of Imperatae Rhizoma had different protective effect in rats with adriamycin nephrosis, and the effect of ethyl acetate group was more stronger than that of other groups. The mechanism may be related to reducing the expression of NF-κB p65 and TGF-ß1, and the content of TNF-α inrenal tissues of rats.