Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China.
J Vet Sci
; 17(4): 523-529, 2016 Dec 30.
Article
en En
| MEDLINE
| ID: mdl-27456776
ABSTRACT
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Enfermedades de las Ovejas
/
Enfermedades de las Cabras
/
Anaplasma
/
Anaplasmosis
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
J Vet Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China