A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research.
G3 (Bethesda)
; 6(10): 3017-3026, 2016 10 13.
Article
en En
| MEDLINE
| ID: mdl-27527793
ABSTRACT
Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biología Evolutiva
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Elementos de Facilitación Genéticos
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Proteínas de Drosophila
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Drosophila
Límite:
Animals
Idioma:
En
Revista:
G3 (Bethesda)
Año:
2016
Tipo del documento:
Article