How can we reduce costs of solid-phase multiplex-bead assays used to determine anti-HLA antibodies?
HLA
; 88(3): 110-9, 2016 09.
Article
en En
| MEDLINE
| ID: mdl-27534609
ABSTRACT
Solid-phase multiplex-bead assays are widely used in transplantation to detect anti-human leukocyte antigen (HLA) antibodies. These assays enable high resolution detection of low levels of HLA antibodies. However, multiplex-bead assays are costly and yield variable measurements that limit the comparison of results between laboratories. In the context of a Dutch national Consortium study we aimed to determine the inter-assay and inter-machine variability of multiplex-bead assays, and we assessed how to reduce the assay reagents costs. Fifteen sera containing a variety of HLA antibodies were used yielding in total 7092 median fluorescence intensities (MFI) values. The inter-assay and inter-machine mean absolute relative differences (MARD) of the screening assay were 12% and 13%, respectively. The single antigen bead (SAB) inter-assay MARD was comparable, but showed a higher lot-to-lot variability. Reduction of screening assay reagents to 50% or 40% of manufacturers' recommendations resulted in MFI values comparable to 100% of the reagents, with an MARD of 12% or 14%, respectively. The MARD of the 50% and 40% SAB assay reagent reductions were 11% and 22%, respectively. From this study, we conclude that the reagents can be reliably reduced at least to 50% of manufacturers' recommendations with virtually no differences in HLA antibody assignments.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
1_ASSA2030
Problema de salud:
1_financiamento_saude
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Inmunoensayo
/
Automatización de Laboratorios
/
Antígenos HLA
/
Isoanticuerpos
Tipo de estudio:
Diagnostic_studies
/
Health_economic_evaluation
Límite:
Humans
Idioma:
En
Revista:
HLA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Países Bajos