Evaluation of Protamine as a Disinfectant for Contact Lenses.
Optom Vis Sci
; 93(11): 1349-1355, 2016 11.
Article
en En
| MEDLINE
| ID: mdl-27536976
ABSTRACT
PURPOSE:
To investigate the ability of protamine, alone or in combination with other antimicrobial agents, to kill bacteria and fungi associated with contact lens-related keratitis.METHODS:
The International Organization for Standardization 147292001 procedure was used to test the antimicrobial activity of solutions of protamine (23-228 µM) with and without polyhexamethylene biguanide (PHMB) and ethylenediamine tetra-acetic acid (EDTA). The recommended ISO panel of microbes along with six clinical isolates was tested. The effect of increasing sodium chloride concentration on the antimicrobial activity was also assessed. The cytotoxicity of the final protamine/EDTA/PHMB solution was measured using ISO 10993-5 standard assays.RESULTS:
Protamine gave a dose-dependent antimicrobial effect, with the highest effect for most strains being at 228 µM (≥6 log reductions of viable bacteria and ≥1 log reduction of viable fungi). Addition of EDTA and PHMB increased the antimicrobial effect for all strains except Pseudomonas aeruginosa ATCC6538, which had optimum activity (≥6 log inhibition) even in protamine alone. The optimum antimicrobial activity of all microbes was achieved in 0.2% sodium chloride, but even in 0.8% sodium chloride, the activity met or exceeded the ISO standard (>3 log reductions for bacteria and >1 log reduction for fungi). None of the formulations was cytotoxic to mammalian cells.CONCLUSIONS:
This study highlights the potential for protamine to be used for the development of effective multipurpose disinfection solutions. Further investigations such as stability, compatibility with contact lenses, and in vivo toxicity are warranted.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
Problema de salud:
2_enfermedades_transmissibles
Asunto principal:
Bacterias
/
Protaminas
/
Lentes de Contacto
/
Desinfectantes
/
Hongos
/
Antiinfecciosos
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Optom Vis Sci
Asunto de la revista:
OPTOMETRIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Australia