Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Cell
; 166(6): 1411-1422.e16, 2016 Sep 08.
Article
en En
| MEDLINE
| ID: mdl-27610567
ABSTRACT
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
ARN Polimerasa II
/
Modelos Moleculares
/
Proteínas de Saccharomyces cerevisiae
/
Complejo Mediador
Idioma:
En
Revista:
Cell
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos