A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma.
Anal Bioanal Chem
; 408(28): 8241-8251, 2016 Nov.
Article
en En
| MEDLINE
| ID: mdl-27655335
ABSTRACT
Heparins are widely used anticoagulant drugs. The current monitoring practice for heparin in plasma, such as the chromogenic anti-factor Xa assay, relies on heparin-triggered activation of antithrombin, an inhibitor of coagulation proteases. Such assays are not applicable to the detection of non-anticoagulant heparins, an emerging class of drug candidates for therapeutic applications unrelated to anticlotting activity. This study describes the application of a commercially available fluorescent probe assay (Heparin Red) for the direct and sensitive detection of the "chemical" heparin in plasma, independent of any anticoagulant activity. The quantification range is about 0-5 µg/mL for both unfractionated heparin (corresponding to 0-1 IU/mL) and the low molecular weight heparin enoxaparin. The Heparin Red assay is of particular value for the quantification of non-anticoagulant heparins, as exemplified by the low molecular weight heparin derivative tafoxiparin and a N-desulfated-N-reacetylated heparin. Heparin octa- and decasaccharides are also detected. Graphical abstract Heparin quantification in plasma by mixing the sample with the Heparin Red reagent and fluorescence readout.
Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Heparina
/
Factor IXa
/
Colorantes Fluorescentes
/
Fluorometría
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
Alemania