Spotsizer: High-throughput quantitative analysis of microbial growth.
Biotechniques
; 61(4): 191-201, 2016 10 01.
Article
en En
| MEDLINE
| ID: mdl-27712582
ABSTRACT
Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https//data.csiro.au/dap/landingpage?pid=csiro15330 under a proprietary CSIRO license.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
Procesamiento de Imagen Asistido por Computador
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Programas Informáticos
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Ensayos Analíticos de Alto Rendimiento
Idioma:
En
Revista:
Biotechniques
Año:
2016
Tipo del documento:
Article
País de afiliación:
Australia