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Alkaline Response of a Halotolerant Alkaliphilic Halomonas Strain and Functional Diversity of Its Na+(K+)/H+ Antiporters.
Cheng, Bin; Meng, Yiwei; Cui, Yanbing; Li, Chunfang; Tao, Fei; Yin, Huijia; Yang, Chunyu; Xu, Ping.
Afiliación
  • Cheng B; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
  • Meng Y; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
  • Cui Y; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
  • Li C; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
  • Tao F; the State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Yin H; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
  • Yang C; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and ycy21th@sdu.edu.cn.
  • Xu P; From the State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100 and.
J Biol Chem ; 291(50): 26056-26065, 2016 Dec 09.
Article en En | MEDLINE | ID: mdl-27777302
Halomonas sp. Y2 is a halotolerant alkaliphilic strain from Na+-rich pulp mill wastewater with high alkalinity (pH >11.0). Transcriptome analysis of this isolate revealed this strain may use various transport systems for pH homeostasis. In particular, the genes encoding four putative Na+/H+ antiporters were differentially expressed upon acidic or alkaline conditions. Further evidence, from heterologous expression and mutant studies, suggested that Halomonas sp. Y2 employs its Na+/H+ antiporters in a labor division way to deal with saline and alkaline environments. Ha-NhaD2 displayed robust Na+(Li+) resistance and high transport activities in Escherichia coli; a ΔHa-nhaD2 mutant exhibited growth inhibition at high Na+(Li+) concentrations at pH values of 6.2, 8.0, and 10.0, suggesting its physiological role in osmotic homeostasis. In contrast, Ha-NhaD1 showed much weaker activities in ion exporting and pH homeostasis. Ha-Mrp displayed a combination of properties similar to those of Mrp transporters from some Bacillus alkaliphiles and neutrophiles. This conferred obvious Na+(Li+, K+) resistance in E. coli-deficient strains, as those ion transport spectra of some neutrophil Mrp antiporters. Conversely, similar to the Bacillus alkaliphiles, Ha-Mrp showed central roles in the pH homeostasis of Halomonas sp. Y2. An Ha-mrp-disrupted mutant was seriously inhibited by high concentrations of Na+(Li+, K+) but only under alkaline conditions. Ha-NhaP was determined to be a K+/H+ antiporter and shown to confer strong K+ resistance both at acidic and alkaline stresses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Intercambiadores de Sodio-Hidrógeno / Halomonas / Antiportadores de Potasio-Hidrógeno Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Intercambiadores de Sodio-Hidrógeno / Halomonas / Antiportadores de Potasio-Hidrógeno Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article
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