Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility.
Infect Dis Obstet Gynecol
; 2016: 5293034, 2016.
Article
en En
| MEDLINE
| ID: mdl-27872553
ABSTRACT
Background. Elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance. Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected. Findings. Group B streptococcus (GBS), Enterococcus faecalis, and Neisseria gonorrhoeae were each detected at 105 bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS and Enterococcus). Sensitivity was increased to 10-2 bacteria/mL for GBS, 10-1 bacteria/mL for E. faecalis, and 101 bacteria/mL for N. gonorrhoeae following 18-24-hour culture. Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Infecciones Bacterianas
/
Pruebas de Sensibilidad Microbiana
/
Tipificación Molecular
/
Antibacterianos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Infect Dis Obstet Gynecol
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
GINECOLOGIA
/
OBSTETRICIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos