A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition.
Stem Cell Res
; 17(2): 401-405, 2016 09.
Article
en En
| MEDLINE
| ID: mdl-27879215
ABSTRACT
Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VEC+ endothelial cells express tdTomato and CD43+ hematopoietic progenitors express eGFP.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
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Antígenos CD
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Cadherinas
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Proteínas Fluorescentes Verdes
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Leucosialina
Límite:
Humans
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Male
Idioma:
En
Revista:
Stem Cell Res
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos