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A human VE-cadherin-tdTomato and CD43-green fluorescent protein dual reporter cell line for study endothelial to hematopoietic transition.
Jung, Ho Sun; Uenishi, Gene; Kumar, Akhilesh; Park, Mi Ae; Raymond, Matt; Fink, Dustin; McLeod, Ethan; Slukvin, Igor.
Afiliación
  • Jung HS; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • Uenishi G; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA.
  • Kumar A; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • Park MA; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • Raymond M; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • Fink D; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • McLeod E; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA.
  • Slukvin I; Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53715, USA; Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53792, USA. Electronic address: islukvin@wisc.edu.
Stem Cell Res ; 17(2): 401-405, 2016 09.
Article en En | MEDLINE | ID: mdl-27879215
ABSTRACT
Human embryonic stem cell line WA01 was genetically modified using zinc-finger nucleases and the PiggyBac/transponson system to introduce a fluorescence reporter for VE-cadherin (VEC; tdTomato) and CD43 (eGFP). Phenotypic and functional assays for pluripotency revealed the modified hES cell reporter lines remained normal. When the cells were differentiated into hematoendothelial lineages, either by directed differentiation or direct reprogramming, flow cytometric and fluorescence microscopy showed that VEC+ endothelial cells express tdTomato and CD43+ hematopoietic progenitors express eGFP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Hematopoyéticas / Antígenos CD / Cadherinas / Proteínas Fluorescentes Verdes / Leucosialina Límite: Humans / Male Idioma: En Revista: Stem Cell Res Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Hematopoyéticas / Antígenos CD / Cadherinas / Proteínas Fluorescentes Verdes / Leucosialina Límite: Humans / Male Idioma: En Revista: Stem Cell Res Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos