Novel PCR-RFLP system based on rpoB gene for differentiation of Cronobacter species.
Food Microbiol
; 62: 1-8, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-27889135
ABSTRACT
Bacteria from the genus Cronobacter are opportunistic foodborne pathogens that can cause severe infections. More rapid, cost-effective and reliable methods are still required for the species identification of Cronobacter spp. In this study, we present a novel PCR-RFLP-based method that uses a newly designed pair of primers for the PCR-amplification of a partial rpoB gene sequence (1635 bp). The amplified products of DNA from 80 Cronobacter strains were separately digested with three restriction endonucleases (Csp6I, HinP1I, MboI). Using the obtained restriction patterns, a PCR-RFLP identification system was created to enable differentiation between all seven currently-known Cronobacter species. The functionality of our method was successfully verified on real food samples. Moreover, the relationships between the Cronobacter species were determined via a phylogenetic tree created from the RFLP patterns.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
ARN Polimerasas Dirigidas por ADN
/
Reacción en Cadena de la Polimerasa
/
Cronobacter
/
Microbiología de Alimentos
/
Genes Bacterianos
Idioma:
En
Revista:
Food Microbiol
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
República Checa