Measuring Ca2+ inside intracellular organelles with luminescent and fluorescent aequorin-based sensors.
Biochim Biophys Acta Mol Cell Res
; 1864(6): 894-899, 2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-27939433
ABSTRACT
GFP-Aequorin Protein (GAP) can be used to measure [Ca2+] inside intracellular organelles, both by luminescence and by fluorescence. The low-affinity variant GAP3 is adequate for ratiometric imaging in the endoplasmic reticulum and Golgi apparatus, and it can be combined with conventional synthetic indicators for simultaneous measurements of cytosolic Ca2+. GAP is bioorthogonal as it does not have mammalian homologues, and it is robust and functionally expressed in transgenic flies and mice, where it can be used for Ca2+ measurements ex vivo and in vivo to explore animal models of health and disease. This article is part of a Special Issue entitled ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Orgánulos
/
Calcio
/
Aequorina
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochim Biophys Acta Mol Cell Res
Año:
2017
Tipo del documento:
Article