Effect of temperature and seven storage media on human periodontal ligament fibroblast viability.
Dent Traumatol
; 33(2): 100-105, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-27976503
ABSTRACT
BACKGROUND/AIM:
Natural resources, such as coconut water, propolis, and egg whites, have been examined as possible storage media for avulsed teeth. However, there is a lack of research focused on the efficacy of these three products together compared with Hank's balanced salt solution and milk. The aim of this study was to evaluate the capacity of seven storage media to maintain the viability of human periodontal ligament fibroblasts (PDLFs). MATERIAL ANDMETHODS:
PDLFs were kept at 5°C and 20°C, in skimmed milk (SMilk), whole milk (WMilk), recently prepared Hank's balanced salt solution (HBSS), Save-A-Tooth® system's HBSS (Save), natural coconut water (Coconut), Propolis, and egg white (Egg) for 3, 6, 24, 48, 72, 96, and 120 h, through the analysis of tetrazolium salt-based colorimetric (MTT) assay.RESULTS:
At 5°C, SMilk and WMilk were better than HBSS in maintaining cell viability, from 24 h onward. At 20°C, HBSS was the best storage medium at 96 and 120 h. At both temperatures, from 6 h onward, Coconut, Propolis and Egg were less effective than SMilk, WMilk, and HBSS. In general, the performance of Coconut, Propolis and Egg were not influenced by storage temperature. However, the lowest temperature undermined the effectiveness of HBSS from 24 h and favored SMilk and WMilk, from 96 and 48 h onward, respectively. Save and water were the worst storage media.CONCLUSION:
SMilk was the best storage medium, followed by WMilk and HBSS. Coconut, Propolis, and Egg can be indicated for the conservation of PDLF up to 3 h. The lower temperature (5°C) undermined the effectiveness of HBSS and favored SMilk and WMilk.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ligamento Periodontal
/
Supervivencia Celular
/
Soluciones Preservantes de Órganos
/
Fibroblastos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Dent Traumatol
Asunto de la revista:
ODONTOLOGIA
/
TRAUMATOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil