Photorhabdusluminescens subsp. namnaonensis subsp. nov., isolated from Heterorhabditisbaujardi nematodes.
Int J Syst Evol Microbiol
; 67(4): 1046-1051, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-28032540
ABSTRACT
A lightly yellowish-pigmented, oxidase-negative bacterial strain (PB45.5T) isolated from the Nam Nao district of Phetchabun in central Thailand was investigated to determine its taxonomic position. Cells of the isolate showed a rod shaped appearance. The strain stained Gram-negative. Strain PB45.5T shared highest 16S rRNA gene sequence similarity with the type strains of Photorhabdus luminescens subsp. akhurstii (99.2â%) and Photorhabdus luminescens subsp. hainanensis (99.1â%) and lower similarities to all other Photorhabdus luminescens subspecies (<98.0â%). Multilocus sequence analysis (MLSA) based on concatenated partial recA, dnaN, gltX, gyrB and infB gene sequences confirmed the affiliation obtained by 16S rRNA gene sequence analysis but showed a clear distinction of PB45.5T from the closest related type strains. Strain PB45.5T shared only 96.9â% sequence similarity (concatenated nucleotide sequences) with P. luminescens subsp. akhurstii FRG04T and 96.8â% with P. luminescens subsp. hainanensis C8404T. The fatty acid profile of the strain consisted of the major fatty acids C14ââ0, C16ââ0, C17ââ0 cyclo, C16ââ1ω7c and/or iso-C15ââ0 2-OH, and C18ââ1ω7c. The MLSA results and the differential biochemical and chemotaxonomic properties showed that strain PB45.5T represents a novel P. luminescens subspecies, for which the name Photorhabdus luminescens subsp. namnaonensis subsp. nov. (type strain PB45.5T=LMG 29915T=CCM 8729T) is proposed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Filogenia
/
Photorhabdus
/
Nematodos
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania