Your browser doesn't support javascript.
loading
Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.
Klementieva, Natalia V; Pavlikov, Anton I; Moiseev, Alexander A; Bozhanova, Nina G; Mishina, Natalie M; Lukyanov, Sergey A; Zagaynova, Elena V; Lukyanov, Konstantin A; Mishin, Alexander S.
Afiliación
  • Klementieva NV; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
  • Pavlikov AI; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
  • Moiseev AA; Institute of Applied Physics, Nizhny Novgorod, Russia.
  • Bozhanova NG; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
  • Mishina NM; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
  • Lukyanov SA; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru and Pirogov Russian National Research Medical University, Moscow, Russia.
  • Zagaynova EV; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
  • Lukyanov KA; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
  • Mishin AS; Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
Chem Commun (Camb) ; 53(5): 949-951, 2017 Jan 10.
Article en En | MEDLINE | ID: mdl-28044165
ABSTRACT
Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Luminiscentes Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Rusia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Luminiscentes Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Rusia