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Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next-Generation Sequencing.
Marosy, Beth A; Craig, Brian D; Hetrick, Kurt N; Witmer, P Dane; Ling, Hua; Griffith, Sean M; Myers, Benjamin; Ostrander, Elaine A; Stanford, Janet L; Brody, Lawrence C; Doheny, Kimberly F.
Afiliación
  • Marosy BA; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Craig BD; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Hetrick KN; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Witmer PD; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Ling H; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Griffith SM; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Myers B; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
  • Ostrander EA; National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland.
  • Stanford JL; Program in Prostate Cancer Research, Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington.
  • Brody LC; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, Maryland.
  • Doheny KF; Center for Inherited Disease Research, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland.
Curr Protoc Hum Genet ; 92: 18.10.1-18.10.25, 2017 01 11.
Article en En | MEDLINE | ID: mdl-28075488
This unit describes a technique for generating exome-enriched sequencing libraries using DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples. Utilizing commercially available kits, we present a low-input FFPE workflow starting with 50 ng of DNA. This procedure includes a repair step to address damage caused by FFPE preservation that improves sequence quality. Subsequently, libraries undergo an in-solution-targeted selection for exons, followed by sequencing using the Illumina next-generation short-read sequencing platform. © 2017 by John Wiley & Sons, Inc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Biblioteca de Genes / Fijación del Tejido / Adhesión en Parafina / Secuenciación de Nucleótidos de Alto Rendimiento / Exoma / Formaldehído / Secuenciación del Exoma Límite: Humans Idioma: En Revista: Curr Protoc Hum Genet Año: 2017 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Biblioteca de Genes / Fijación del Tejido / Adhesión en Parafina / Secuenciación de Nucleótidos de Alto Rendimiento / Exoma / Formaldehído / Secuenciación del Exoma Límite: Humans Idioma: En Revista: Curr Protoc Hum Genet Año: 2017 Tipo del documento: Article