The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.

Kalms, Jacqueline; Banthiya, Swathi; Galemou Yoga, Etienne; Hamberg, Mats; Holzhutter, Hermann-Georg; Kuhn, Hartmut; Scheerer, Patrick

Kalms, Jacqueline; Banthiya, Swathi; Galemou Yoga, Etienne; Hamberg, Mats; Holzhutter, Hermann-Georg; Kuhn, Hartmut; Scheerer, Patrick.

Afiliación

Kalms J; Institute of Medical Physics and Biophysics (CC2), Group Protein X-ray Crystallography and Signal Transduction, Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Banthiya S; Institute for Biochemistry (CC2), Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Galemou Yoga E; Institute of Medical Physics and Biophysics (CC2), Group Protein X-ray Crystallography and Signal Transduction, Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Hamberg M; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Holzhutter HG; Institute for Biochemistry (CC2), Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Kuhn H; Institute for Biochemistry (CC2), Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: hartmut.kuehn@charite.de.
Scheerer P; Institute of Medical Physics and Biophysics (CC2), Group Protein X-ray Crystallography and Signal Transduction, Charité - University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany. Electronic address: patrick.scheerer@charite.de.

Biochim Biophys Acta Mol Cell Biol Lipids ; 1862(5): 463-473, 2017 May.

Article en En | MEDLINE | ID: mdl-28093240

Asunto(s)

Araquidonato 15-Lipooxigenasa/química; Ácido Araquidónico/metabolismo; Proteínas Mutantes/química; Oxígeno/metabolismo; Araquidonato 15-Lipooxigenasa/genética; Araquidonato 15-Lipooxigenasa/metabolismo; Ácido Araquidónico/química; Sitios de Unión; Catálisis; Cristalografía por Rayos X; Modelos Moleculares; Mutagénesis Sitio-Dirigida; Proteínas Mutantes/genética; Oxígeno/química; Conformación Proteica; Pseudomonas aeruginosa/enzimología; Relación Estructura-Actividad; Especificidad por Sustrato

Palabras clave

Catalysis; Eicosanoids; Mutagenesis; Oxidative stress; Oxygen sensor

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Araquidonato 15-Lipooxigenasa / Ácido Araquidónico / Proteínas Mutantes Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2017 Tipo del documento: Article País de afiliación: Alemania

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google