Mass Spectrometry: A Technique of Many Faces.
Q Rev Biophys
; 492016 Jan.
Article
en En
| MEDLINE
| ID: mdl-28100928
ABSTRACT
Protein complexes form the critical foundation for a wide range of biological process, however understanding the intricate details of their activities is often challenging. In this review we describe how mass spectrometry plays a key role in the analysis of protein assemblies and the cellular pathways which they are involved in. Specifically, we discuss how the versatility of mass spectrometric approaches provides unprecedented information on multiple levels. We demonstrate this on the ubiquitin-proteasome proteolytic pathway, a process that is responsible for protein turnover. We follow the various steps of this degradation route and illustrate the different mass spectrometry workflows that were applied for elucidating molecular information. Overall, this review aims to stimulate the integrated use of multiple mass spectrometry approaches for analyzing complex biological systems.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
Límite:
Humans
Idioma:
En
Revista:
Q Rev Biophys
Año:
2016
Tipo del documento:
Article
País de afiliación:
Israel