Cell-controlled hybrid perfusion fed-batch CHO cell process provides significant productivity improvement over conventional fed-batch cultures.
Biotechnol Bioeng
; 114(7): 1438-1447, 2017 07.
Article
en En
| MEDLINE
| ID: mdl-28128436
ABSTRACT
A simple method originally designed to control lactate accumulation in fed-batch cultures of Chinese Hamster Ovary (CHO) cells has been modified and extended to allow cells in culture to control their own rate of perfusion to precisely deliver nutritional requirements. The method allows for very fast expansion of cells to high density while using a minimal volume of concentrated perfusion medium. When the short-duration cell-controlled perfusion is performed in the production bioreactor and is immediately followed by a conventional fed-batch culture using highly concentrated feeds, the overall productivity of the culture is approximately doubled when compared with a highly optimized state-of-the-art fed-batch process. The technology was applied with near uniform success to five CHO cell processes producing five different humanized monoclonal antibodies. The increases in productivity were due to the increases in sustained viable cell densities. Biotechnol. Bioeng. 2017;114 1438-1447. © 2017 Wiley Periodicals, Inc.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Perfusión
/
Células CHO
/
Ácido Láctico
/
Proliferación Celular
/
Técnicas de Cultivo Celular por Lotes
/
Glucosa
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Bioeng
Año:
2017
Tipo del documento:
Article