Photometric Determination of Ammonium and Phosphate in Seawater Medium Using a Microplate Reader.
J Mol Microbiol Biotechnol
; 27(2): 73-80, 2017.
Article
en En
| MEDLINE
| ID: mdl-28171869
ABSTRACT
To more efficiently process the large sample numbers for quantitative determination of ammonium (NH4+) and phosphate (orthophosphate, PO43-) generated during comprehensive growth experiments with the marine Roseobacter group member Phaeobacter inhibens DSM 17395, specific colorimetric assays employing a microplate reader (MPR) were established. The NH4+ assay is based on the reaction of NH4+ with hypochlorite and salicylate, yielding a limit of detection of 14 µM, a limit of quantitation of 36 µM, and a linear range for quantitative determination up to 200 µM. The PO43-assay is based on the complex formation of PO43- with ammonium molybdate in the presence of ascorbate and zinc acetate, yielding a limit of detection of 13 µM, a limit of quantitation of 50 µM, and a linear range for quantitative determination up to 1 mM. Both MPR-based assays allowed for fast (significantly lower than 1 h) analysis of 21 samples plus standards for calibration (all measured in triplicates) and showed only low variation across a large collection of biological samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatos
/
Fotometría
/
Agua de Mar
/
Medios de Cultivo
/
Compuestos de Amonio
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Mol Microbiol Biotechnol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
/
MICROBIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania