Comparison of four molecular approaches to identify Candida parapsilosis complex species.
Mem Inst Oswaldo Cruz
; 112(3): 214-219, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-28225905
ABSTRACT
Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Candida
/
Técnicas de Tipificación Micológica
Límite:
Humans
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil