An expression vector inhibits gene expression in Xenopus embryos by antisense RNA.
Rouxs Arch Dev Biol
; 201(6): 340-345, 1992 Oct.
Article
en En
| MEDLINE
| ID: mdl-28305852
ABSTRACT
An expression vector was constructed containing the entire bovine papilloma virus (BPV-1) genome and part of the a-actin gene of Xenopus laevis cloned in the antisense orientation into the neomycin resistance gene under the control of the herpes simplex virus (HSV) thymidine kinase (TK) promoter. When this vector is microinjected into X. laevis embryos it replicates extrachromosomally, at least up to the tadpole stage, and a fusion RNA is synthesized after the mid blastula transition (MBT). The expression of the antisense gene results in a morphological abnormality of somites demonstrating that antisense RNA generated by an episomal replicating expression vector can inhibit the expression of a selected gene during early embryogenesis of X. laevis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Rouxs Arch Dev Biol
Asunto de la revista:
BIOLOGIA
/
EMBRIOLOGIA
Año:
1992
Tipo del documento:
Article
País de afiliación:
Alemania