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A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.
Alimolaei, Mojtaba; Golchin, Mehdi; Abshenas, Jalil; Ezatkhah, Majid; Bafti, Mehrdad Shamsaddini.
Afiliación
  • Alimolaei M; Department of Molecular Microbiology, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
  • Golchin M; Department of Anaerobic Bacterial Vaccine Research and Production, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
  • Abshenas J; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran.
  • Ezatkhah M; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, Kerman, 7636161313, Iran. golchin@uk.ac.ir.
  • Bafti MS; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Probiotics Antimicrob Proteins ; 10(2): 251-257, 2018 06.
Article en En | MEDLINE | ID: mdl-28401477
The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-α strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy. BALB/c mice, immunized orally by the recombinant LC-α strain, elicited mucosal and significantly humoral immune responses (p < 0.05) and developed a protection against 900 MLD/mL of the standard alpha-toxin. This study showed that this recombinant LC-α strain could be a promising vaccine candidate against gas gangrene and necrotic enteritis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 Problema de salud: 2_enfermedades_transmissibles Asunto principal: Toxoides / Vacunas Bacterianas / Clostridium perfringens / Probióticos / Enteritis / Gangrena Gaseosa / Lacticaseibacillus casei Límite: Animals / Female / Humans Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2018 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 2_ODS3 Problema de salud: 2_enfermedades_transmissibles Asunto principal: Toxoides / Vacunas Bacterianas / Clostridium perfringens / Probióticos / Enteritis / Gangrena Gaseosa / Lacticaseibacillus casei Límite: Animals / Female / Humans Idioma: En Revista: Probiotics Antimicrob Proteins Año: 2018 Tipo del documento: Article País de afiliación: Irán
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