The role of STAT3 and AhR in the differentiation of CD4+ T cells into Th17 and Treg cells.
Medicine (Baltimore)
; 96(17): e6615, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-28445259
ABSTRACT
BACKGROUND:
This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4 T cells into T helper (Th)17 and T regulatory (Treg) cells.METHODS:
First, CD4 T cells were isolated from the spleen of BALB/c mice. Then, stable CD4 T cells expressing STAT3 shRNA were constructed. CD4 T cells were assigned to one of the following treatments Th17 group antibodies against CD3 and CD28, 2.5âng/mL transforming growth factor ß (TGF-ß)1, 30âng/mL interleukin (IL)-6, and 30âng/mL IL-23; 6-formylindolo[3,2-b]carbazole (FICZ) group antibodies against CD3 and CD28, 2.5âng/mL TGF-ß1, 30âng/mL IL-6, 30âng/mL IL-23, and 100ânM FICZ; FICZ + STAT3 RNAi group (shSTAT3 group) antibodies against CD3 and CD28, 2.5âng/mL TGF-ß1, 30âng/mL IL-6, 30âng/mL IL-23, 100ânM FICZ, and STAT3 RNAi; naphthoflavone group antibodies against CD3 and CD28, 2.5âng/mL TGF-ß1, 30âng/mL IL-6, 30âng/mL IL-23, and 3 µM naphthoflavone; 5) no antibodies were added in the control group. Later, the proportions of Th17 and Treg cells in each group were measured by flow cytometry; phospho-STAT3 and -STAT5 levels were measured by western blotting; and AhR, STAT3, STAT5, receptor-related orphan nuclear receptor γt (RORγt), FOXP3, T-cell receptor (TCR), CD25, IL-6R, IL-10, and IL-17 mRNA levels were also measured by real-time PCR.RESULT:
Th17 cells showed a rise and Treg cells showed a decrease in the FICZ group, but revised in the shSTAT3 group and the naphthoflavone group. Significant differences were observed in CD25, IL-6R, IL-10, and IL-17 mRNA levels among different groups.CONCLUSION:
STAT3 may cooperate with AhR to regulate the differentiation of both Th17 and Treg cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos CD4
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Diferenciación Celular
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Linfocitos T Reguladores
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Receptores de Hidrocarburo de Aril
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Factor de Transcripción STAT3
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Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
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Células Th17
Límite:
Animals
Idioma:
En
Revista:
Medicine (Baltimore)
Año:
2017
Tipo del documento:
Article
País de afiliación:
China