DNA fluorescent labeling with naphtho[1,2,3-cd]indol-6(2H)-one for investigation of protein-DNA interactions.
Bioorg Chem
; 72: 268-272, 2017 06.
Article
en En
| MEDLINE
| ID: mdl-28495557
ABSTRACT
Fluorescently labeled DNA to study protein-DNA interactions was synthesized using the Cu(I)-catalysed cycloaddition (CuAAC) reaction. For this purpose, a new azido-containing fluorophore based on the naphtho[1,2,3-cd]indol-6(2H)-one derivative was obtained. The fluorescent properties of naphtho[1,2,3-cd]indol-6(2H)-one derivatives and labeled DNA were studied. The new fluorescent DNA conjugate was shown to be a useful tool to study complex mechanisms of protein-DNA interactions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
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ADN Glicosilasas
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Fluorescencia
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Colorantes Fluorescentes
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Indoles
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Naftalenos
Límite:
Humans
Idioma:
En
Revista:
Bioorg Chem
Año:
2017
Tipo del documento:
Article