Study of RNA-A Initiation Translation of The Infectious Pancreatic Necrosis Virus.
Virus Res
; 240: 121-129, 2017 08 15.
Article
en En
| MEDLINE
| ID: mdl-28743463
ABSTRACT
The infectious pancreatic necrosis virus (IPNV) is a salmonid pathogen that causes significant economic losses to the aquaculture industry. IPNV is a non-enveloped virus containing two uncapped and non-polyadenylated double strand RNA genomic segments, RNA-A and RNA-B. The viral protein Vpg is covalently attached to the 5' end of both segments. There is little knowledge about its viral cycle, particularly about the translation of the RNAs. Through experiments using mono and bicistronic reporters, in this work we show that the 120-nucleotide-long 5'-UTR of RNA-A contains an internal ribosome entry site (IRES) that functions efficiently both in vitro and in salmon cells. IRES activity is strongly dependent on temperature. Also, the IRES structure is confined to the 5'UTR and is not affected by the viral coding sequence. This is the first report of IRES activity in a fish virus and can give us tools to generate antivirals to attack the virus without affecting fish directly.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
ARN Viral
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Virus de la Necrosis Pancreática Infecciosa
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Infecciones por Birnaviridae
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Enfermedades de los Peces
Límite:
Animals
Idioma:
En
Revista:
Virus Res
Asunto de la revista:
VIROLOGIA
Año:
2017
Tipo del documento:
Article