An enzymatic strategy to asymmetrically branched N-glycans.
Org Biomol Chem
; 15(35): 7258-7262, 2017 Sep 13.
Article
en En
| MEDLINE
| ID: mdl-28832049
ABSTRACT
An enzymatic strategy was developed to generate asymmetrically branched N-glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ ß-galactosidase was employed to selectively trim symmetrically branched N-glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Glicosiltransferasas
/
Beta-Galactosidasa
Límite:
Animals
Idioma:
En
Revista:
Org Biomol Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos