Metabolomic-Guided Isolation of Bioactive Natural Products from Curvularia sp., an Endophytic Fungus of Terminalia laxiflora.
Planta Med
; 84(3): 182-190, 2018 Feb.
Article
en En
| MEDLINE
| ID: mdl-28847019
ABSTRACT
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-κB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using high-resolution electrospray ionization mass spectrometry directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1 ) and two linear peptide congeners of 1 dipeptide N-acetylphenylalanyl-L-phenylalanine (2 ) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3 ).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ascomicetos
/
Productos Biológicos
/
Terminalia
Límite:
Humans
Idioma:
En
Revista:
Planta Med
Año:
2018
Tipo del documento:
Article
País de afiliación:
Reino Unido