Synthesis of a thiophosphate analog of dioctanoylphosphatidylcholine: a phospholipase C substrate.
J Lipid Res
; 28(8): 949-54, 1987 Aug.
Article
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| MEDLINE
| ID: mdl-2889786
ABSTRACT
Dioctanoylthiophosphatidylcholine, a racemic thiophosphate analog of L-alpha-dioctanoylphosphatidylcholine, has been synthesized and isolated by flash chromatography. In contrast with the didecanoylthiophosphatidylcholine synthesized previously, the analog is easily dispersed on sonication in aqueous media and is rapidly hydrolyzed to produce a free thiol group in the presence of the extracellular phospholipase C from either Bacillus cereus or Clostridium perfringens. When 5,5'-dithiobis (2-nitro-benzoic acid) was included as a thiol reactive chromogenic agent, the resultant measurement of product release, as an increase in absorbance at 412 nm, showed a linear relationship with added enzyme.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Fosfolipasas de Tipo C
Idioma:
En
Revista:
J Lipid Res
Año:
1987
Tipo del documento:
Article