Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.
Methods Enzymol
; 597: 145-186, 2017.
Article
en En
| MEDLINE
| ID: mdl-28935101
ABSTRACT
This method describes the chemoenzymatic synthesis of several nucleotide sugars, which are essential substrates in the biosynthesis of prokaryotic N- and O-linked glycoproteins. Protein glycosylation is now known to be widespread in prokaryotes and proceeds via sequential action of several enzymes, utilizing both common and modified prokaryote-specific sugar nucleotides. The latter, which include UDP-hexoses such as UDP-diNAc-bacillosamine (UDP-diNAcBac), UDP-diNAcAlt, and UDP-2,3-diNAcManA, are also important components of other bacterial and archaeal glycoconjugates. The ready availability of these "high-value" intermediates will enable courses of study into inhibitor screening, glycoconjugate biosynthesis pathway discovery, and unnatural carbohydrate incorporation toward metabolic engineering.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Azúcares de Uridina Difosfato
/
Carbohidratos
/
Glicoconjugados
/
Ingeniería Metabólica
Idioma:
En
Revista:
Methods Enzymol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos