Desmin expression profile in reactive astrocytes in the 3-nitropropionic acid-lesioned striatum of rat: Characterization and comparison with glial fibrillary acidic protein and nestin.
Acta Histochem
; 119(8): 795-803, 2017 Oct.
Article
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| MEDLINE
| ID: mdl-29054283
ABSTRACT
Desmin, a muscle-specific, type-III intermediate-filament protein, is reportedly expressed in astrocytes in the central nervous system. These cells become reactive astrocytes in response to brain injuries. To elucidate whether desmin is involved in this process, we examined the spatiotemporal expression profiles of desmin and their relationship with two astroglial intermediate filaments, glial fibrillary acidic protein (GFAP) and nestin, in the striatum of rats treated with the mitochondrial toxin 3-nitropropionic acid (3-NP). Weak, constitutive immunoreactivity for desmin was observed in astrocytes generally, and in reactive astrocytes in the peri-lesional area, its expression increased in parallel with that of GFAP over 3 d post-lesion and was maintained until at least day 28. Desmin, GFAP, and nestin showed characteristic time-dependent expression patterns in reactive astrocytes forming the astroglial scar; delayed and long-lasting induction of desmin and GFAP, and rapid but transient induction of nestin. In the lesion core, desmin was expressed in two categories of perivascular cells nestin-negative and nestin-positive. These findings show that desmin, together with GFAP and nestin, is a dynamic component of intermediate filaments in activated astroglia, which may account for the dynamic structural changes seen in these cells in response to brain injuries.
Palabras clave
3,3'-diaminobenzidine tetrahydrochloride; 3-NP; 3-nitropropionic acid; 4',6-diamidino-2'-phenylindole; ANOVA; CNS; DAB; DAPI; Desmin; GFAP; Glial fibrillary acidic protein; Glial scar; LV; Nestin; PB; RECA1; Reactive astrocytes; SDS; SEM; analysis of variance; central nervous system; endothelial cell marker; glial fibrillary acidic protein; i.p.; intraperitoneally; lateral ventricle; phosphate buffer; sodium dodecyl sulfate; standard error of the mean
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Astrocitos
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Cuerpo Estriado
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Desmina
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Nestina
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Proteína Ácida Fibrilar de la Glía
Límite:
Animals
Idioma:
En
Revista:
Acta Histochem
Año:
2017
Tipo del documento:
Article