A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330.
Carbohydr Polym
; 179: 10-18, 2018 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-29111031
ABSTRACT
A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691s-1 and a half-life time of 111h at 30°C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1×109g·mol-1 that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Dextranos
/
Oenococcus
/
Glucosiltransferasas
Idioma:
En
Revista:
Carbohydr Polym
Año:
2018
Tipo del documento:
Article
País de afiliación:
Francia