One-pot synthesis and characterization CdTe:Zn2+ quantum dots and its molecular interaction with calf thymus DNA.

ABSTRACT

Tremendous research efforts have been dedicated to fabricating high-quality Zn-doped CdTe quantum dots (QDs) for any potential biomedical applications. In particular, the correlation of issues regarding how QDs interact with DNA is of greatest importance. Herein, a pH-responsive study of the interactions between CdTeZn2+ quantum dots with 4 different sizes and calf thymus DNA (ctDNA) was conducted using multispectroscopic techniques and electrochemical investigation. Fluorescence studies revealed that this interaction process is predominantly a static process and groove binding was the main binding mode for CdTeZn2+ QDs to ctDNA. The calculated negative values of enthalpy (-45.06 kJ mol-1 ) and entropy (-133.62 J mol-1  K-1 ) with temperature changes indicated that the hydrogen bonds and van der Waals interactions played major roles in the reaction. Furthermore, circular dichroism spectroscopy and Fourier transform infrared spectrometry analyses indicate that the normal conformation of ctDNA is discombobulated by CdTeZn2+ QDs. In addition, the electrochemical behavior of the affinity of CdTeZn2+ QDs for ctDNA agreed well with the results obtained from fluorescence experiments. This study might be meaningful for understanding the molecular binding mechanism of QDs for DNA and provides a basis for QD-labeled systems.