The Effect of Various Stabilizers on Preserving Immunogenicity of Lyophilized Mumps Vaccines.
J Res Health Sci
; 17(4): e00393, 2017 Sep 12.
Article
en En
| MEDLINE
| ID: mdl-29233955
ABSTRACT
BACKGROUND:
Chemical stabilizers are added to live attenuated vaccines for enhancing the virus stability. The aim of this study was to evaluate the effect of various stabilizers on preserving immunogenicity of lyophilized mumps vaccines. STUDYDESIGN:
An experimental study.METHODS:
Three mumps vaccines with different formulations were inoculated to three groups of Guinea pigs. Sterile water was injected to eight Guinea pigs as a control group. Blood samples were collected before inoculation and on 14, 28 and 42 d after vaccine injection. Mumps antibodies in the sera were measured using hemagglutination inhibition assay (HAI).RESULTS:
All three formulated mumps vaccines induced antibody in Guinea pigs after two weeks. Formulation 1 containing trehalose dihydrate and formulation 2 comprised human serum albumin stimulated antibodies in the higher level than Razi routine formulation.CONCLUSIONS:
Various stabilizers have different preservation potencies that differently affect immune response against virus. More stable and more immunogenic vaccines can be produced using stabilizers containing trehalose dihydrate.Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
Problema de salud:
2_enfermedades_transmissibles
Asunto principal:
Trehalosa
/
Vacuna contra la Parotiditis
/
Vacunación
/
Paperas
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
J Res Health Sci
Año:
2017
Tipo del documento:
Article
País de afiliación:
Irán