Cell-free Generation of COPII-coated Procollagen I Carriers.
Bio Protoc
; 7(22)2017 Nov 20.
Article
en En
| MEDLINE
| ID: mdl-29276723
ABSTRACT
The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This protocol describes the preparation of donor membrane and cytosol, the assembly of the reaction, and the isolation and detection of reconstituted COPII-coated carriers. This cell-free reaction can be used to test conditions that stimulate or suppress the packaging of PC1 into COPII-coated carriers.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos