miR985p promotes osteoblast differentiation in MC3T3E1 cells by targeting CKIP1.
Mol Med Rep
; 17(3): 4797-4802, 2018 03.
Article
en En
| MEDLINE
| ID: mdl-29328483
ABSTRACT
Casein kinase 2-interacting protein 1 (CKIP-1) is a negative regulator for bone formation. Previously, using bioinformatics analysis, CKIP1 has been predicted to serve the role of target gene of miR985p. In the present study, the potential role of miR985p in regulating osteoblast differentiation through CKIP1 was investigated. Following pretreatment with microRNA (miR)985p agomir or miR985p antagomir, MC3T3E1 cells were cultured in an osteoinductive medium. Subsequently, the expression of miR985p, CKIP1 and levels of osteoblast differentiation markers, including alkaline phosphatase, matrix mineralization, osteocaicin, collagen type I, runtrelated transcription factor 2 and osteopontin were assayed. Using a dualluciferase reporter assay, it was demonstrated that CKIP1 was the target gene of miR985p. miR985p was upregulated as a result of treatment with miR985p agomir and promoted osteoblast differentiation. Conversely, miR985p antagomir inhibited miR985p expression and osteoblast differentiation. miR985p targeted CKIP1 by binding to its 3'untranslated region. Furthermore, miR985p overexpression decreased the protein levels of CKIP1 and inhibition of miR985p increased the protein levels of CKIP1. The results of the present study indicated that CKIP1 was a target gene of miR985p and that miR985p regulated osteoblast differentiation in MC3T3E1 cells by targeting CKIP1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
MicroARNs
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article